Figure 4
From: A hydrophilic gel matrix for single-molecule super-resolution microscopy
Super-resolution imaging of histone H2B and nascent DNA at different axial planes. Replicated DNA (EdU-Alexa Fluor 647, magenta) and H2B (mEos2-H2B, green) were imaged (a) at the bottom and (d) at the middle of a HeLa cell. The nascent DNA shows the typical distribution for a mid S phase nucleus. A magnified view of selected regions (white boxes, (b, e)) highlights that the distribution of DNA and H2B is different at this stage of the cell cycle. (c, f) Fiducial markers which were not used for correction of drift and chromatic offset are colocalized and demonstrate the quality of image registration (scale bars 5 μm (a, d), 500 nm (b, c, e, f)).