Figure 5
From: Dynamic fluorescence imaging of the free radical products of X-ray absorption in live cells
Average cellular fluorescence intensity in MEF cells over the time course of 7 minutes. The data from the four experiments are graphed. The Fluorescence intensities were obtained from time series (Additional file 2) taken on the modified confocal microscope (Figure 1) at 30 second intervals. The MEF cells were loaded with the fluorescent ROS sensor H2DCFDA. The X-ray tube was turned on after the 5th image and stayed on till the end of the series. Cells were kept at room temperature (21 °C) during imaging. The fluorescence intensity was nearly constant before radiation, immediately rose upon onset of the x-ray beam, and increased at accelerating pace over the course of radiation. The radiation dose rate was 3.0 Gy/min.