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Optical Nanoscopy
Figure 1 | Optical Nanoscopy

Figure 1

From: SOFI-based 3D superresolution sectioning with a widefield microscope

Figure 1

Lateral cross-sections. Subset of images recorded along the optical axis (in steps of 1.2um, starting with e lowest slice on the left side). Upper panels: Original widefield fluorescence images Lower panels: SOFI images. The optical sectioning capabilities are evident as well as the resolution gain along x-y. The SOFI images were processed using 2000 frames per z-position. Fluorescence images are representing the average of 2000 frames. Scalebar 20 μm.

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