Figure 5
Light sheet Bayesian microscopy reveals the distribution of heterochromatin protein HP1α in an H1 cell. (a) Frame-averaged conventional fluorescent image of the distribution of mEos-tagged heterochromatic protein HP1α in a hESC (H1). Green dots are localizations generated by quickPALM using the same imaging data. (b) Bayesian reconstructed super-resolution image of the same region demonstrating distinct regions of HP1α. Inset panels contrast marked difference in resolution. (c) Schematic illustration of the light sheet sectioning at various depths of the nucleus. (d)-(i) Sections of super-resolution images of HP1α in the nucleus of an H1 cell. Conserved topographical structures were observed at different planes 600-nm apart in the axial direction.