Figure 4

Bayesian reconstruction of microtubule fibers. (a) Conventional fluorescent image of the microtubules in a fixed U2OS cell. (b) Bayesian reconstruction of the superresolution image of (a) obtained from 642-nm light sheet illumination. (c) High-magnification view of the highlighted regions in (a) (upper panel) and (b) (lower panel). (d) Cross profile for fiber sections outlined in the lower panel in (c). The light sheet Bayesian microscope resolves 20-nm fibers separated by a distance of 30 nm, demonstrating its lateral resolution of 20-30 nm with fixed samples.