Figure 3From: Light-sheet Bayesian microscopy enables deep-cell super-resolution imaging of heterochromatin in live human embryonic stem cells Light sheet illumination provides enhanced SNR for single-molecule imaging in the nucleus. (a) Schematic depiction of the light-sheet and epi-illumination. (b) Single-molecule images collected from light-sheet (top) and epi-illumination (bottom) of the nucleus of fixed Hues6 (hESC) cells expressing mEos3 on HP1α. (c) Cross profiles demonstrating the enhanced signal-to-noise ration of the single-molecule event from light-sheet (top) vs. epi-illumination (bottom).Back to article page