Skip to main content
Figure 2 | Optical Nanoscopy

Figure 2

From: Light-sheet Bayesian microscopy enables deep-cell super-resolution imaging of heterochromatin in live human embryonic stem cells

Figure 2

Characterization of the light sheet profile. (a) Electromagnetic simulation of the intensity of a focused Gaussian beam at the interface. (b) Width and length of the light sheet at various incident angles at the interface calculated from the intensity profile obtained from electromagnetic simulations. Light-sheet width was calculated from the FWHM of the intensity profile, light-sheet length was calculated from the glass-water interface along the light sheet to the point where its intensity drops by one half. Glass prism with a refractive index of 1.515 and water with a refractive index of 1.333 were used for the simulation at 561 nm. (c) Side view of the light sheet focused at the interface where it enters water. (d) Side profile of the light sheet focused in water, i.e. further away from the interface, and the measurement of its thickness from the cross line profile.

Back to article page