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Figure 3 | Optical Nanoscopy

Figure 3

From: Nanoscale elucidation of Na,K-ATPase isoforms in dendritic spines

Figure 3

Nanoscopic resolution of Na,K-ATPase pumps in spines with PALM using PAGFP and PSD-95 synaptic labeling. (a) Wide-field image of neuron dendrite expressing PSD-95 tagged with mCherry (red) to identify postsynaptic regions. (b) Constructed PALM image of PAGFP-tagged sodium pumps in rainbow color scale showing heterogeneous expression in dendrites and spines. The red line shows an outline of the dendrite branch constructed from a time projection of wide-field PAGFP images. (c) PALM image in gray scale overlaid with the corresponding PSD-95 image from (a). Scale bar = 500 nm.

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