Comparison of fluorescent beads imaged in OPRA (a) and widefield (b) mode (with an arrested scan system). For determination of the FWHM, 8 beads were individually fitted with a 2D Gaussian function. The data shown in (c) corresponds to the average of 3 consecutive centered lines of the summed 8 bead images. Solid lines in (c) correspond to the average FWHM results. The FWHM of the scanned bead images is visibly reduced. The average FWHMs of the 8 beads in the respective images were determined to amount to 473 ± 19 nm (widefield) and 327 ± 4 nm (OPRA). Scale bar 5μm.