Figure 3

Drift correction and registration of super-resolution images of cells. Cells were treated with the thymidine analogue EdU for 15 minutes, DNA was labeled with both Alexa Fluor 647 (magenta) and Alexa Fluor 488 (green) using click chemistry. HeLa cells were imaged in mid stage (a, b) and early stage (c, d), both original images (a, c) and corrected images (b, d) are shown (drift and spectral offset was increased for demonstration purpose in (a, c)). The corrected images (b, d) show colocalization of DNA labeled with two fluorophores (scale bars 5 μm).