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Figure 1 | Optical Nanoscopy

Figure 1

From: A hydrophilic gel matrix for single-molecule super-resolution microscopy

Figure 1

Sample embedding in hydrophilic matrix gel. (a) Fiducial markers can be distributed in an ECM gel in 3D space, and can be used for correction of drift and channel offsets in super-resolution microscopy. The distribution in 3D allows recording images in different focal planes; a sufficient density of the same markers in two imaging planes allows 3D registration of super-resolution images. (b) The positions of four fiducial markers distributed in the ECM gel were tracked in 3D for an acquisition of 10.000 frames, and are color-coded for the axial position. After drift correction, a localization precision of 6.9 nm (s.d., lateral) and 18.1 nm (axial) was determined (scale bars 100 nm).

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