Co-localization between hDcp1a ((b) and (e)) and tubulin ((c) and (f)) in a HeLa cell, presented as a FFFM (a) and a SOFI overlay (d). Tubulin and Dcp1a were labeled with 800 nm and 625 nm QDs and are false-colored in red and green in (a) and (d), respectively. Lower panels: Cross-sections along the lines as indicated in the upper images. On the left: intensity profile of the region indicated by the red line in the FFFM (b) (red solid line) and the SOFI image (e) (red dashed line) of hDcp1a labeled by 625-nm QDs. On the right: intensity profile of the region indicated by the blue line in the FFFM (c) (blue solid line) and the SOFI image (f) (blue dashed line) of alpha-tubulin labeled by 800-nm QDs. Scale bar: (—) = 5.0 μm.