FFFM images ((a), (b) and (c)) of a U2OS cell treated with puromycin: (a) exogenous GFP-hDcp1a protein emission (green); (b) 625-nm QD anti-hDcp1a emission (red); and (c) co-localization of GFP and 625-QD. Co-localization of exogenous GFP-hDcp1a and endogenous hDcp1a marked by 625-QD confirms the selectivity of the QD conjugate. Images do not reveal only the presence of PBs, distinguishable by their typical punctate appearance, but also a wide distribution of hDcp1a proteins all over the cytoplasm, noticeable observing either GFP (a) or QD (b) emission. Indeed, Dcp1a is located in both cytosol and PBs. (d) another FFFM of 625-nm QD anti-hDcp1a, and (e) correspoding SOFI image. Scale bar=5.0 μm.