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Optical Nanoscopy

Table 1 Photoconversion efficiencies and colocalization fractions for the three fusion constructs investigated in this work

From: Identification of the factors affecting co-localization precision for quantitative multicolor localization microscopy

Fused fluorophore pair

First fluorophore efficiency

Second fluorophore efficiency

Colocalization fraction (% of minority species)

Dronpa-mEos2

n.d.

53%

13%

PSCFP2-mEos2

23%

53%

0%

PSCFP2-PAMCherry1

28%

58%

15%

  1. Colocalization fraction is defined as the number of fluorophore pairs falling within a 200 nm radius in a diluted sample. For constructs with mEos2 colocalization fraction will depend on the number of localized mEos2 molecules.
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