(a) DIC image of HeLa cell and overlay of red channel PALM image showing clathrin coated pits on the plasma membrane. (b) and (c) TIRF green and red channels of the selected region in (a) (d) dual color TIRF image of the region selected in a. (e) raw dual color PALM image of the region corresponding to (d). (f) Dual color PALM image of the clustered fraction only (g) and (h) show a detail of a colocalization event between a clathrin coated pit and a β2-AR cluster in TIRF and PALM (clustered fraction) respectively (i) localized map of the position of each fluorophore in both channel corresponding to (f). Scale bar is 1 μm.