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Figure 3 | Optical Nanoscopy

Figure 3

From: Dynamic fluorescence imaging of the free radical products of X-ray absorption in live cells

Figure 3

X-ray induced fluorescence increase in 100 μM solutions of de-esterified H2DCFDA, DHF, and de-esterified H2DCFDA + 1 mM ascorbate (free radical scavenger). (a) Time series of fluorescence images were acquired on the modified confocal microscope (Figure 1). The interval between images was 30 seconds. For the first two solutions the x-ray tube was switched on after image No.5, and persisted to the end of the time series. An immediate rise of the fluorescence intensity can be seen in the de-esterified H2DCFDA solution, while a smaller response was seen in the DHF solution. For the de-esterified H2DCFDA + ascorbate solution the fluorescence over 5 minutes of x-ray exposure was recorded. (b) The average increase of fluorescence intensity per unit dose of x-ray exposure was calculated for the 3 solutions from 3 separate samples for each dye. The error bars are standard deviation.

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