bSOFI demonstration. Experimental demonstration of bSOFI on fixed HeLa cells with Alexa647-labelled microtubules. (a) Summed TIRF microscopy image [Widefield]. (b) Conventional 5th order cross-cumulant SOFI [SOFI5]. (c-e) Color-coded molecular on-time ratio, brightness and density overlaid with the 5th order balanced cumulant [BC5]. (f-h) Profiles along the cuts 1-1’, 2-2’ and 3-3’. In yellow we added the corresponding profiles when Fourier reweighting (cf. (Dertinger et al.
2010)) with a damping factor of 5% is applied on the 5th order cross-cumulant SOFI image. (i-k) Magnified views from the white insets highlighted in (a-c). Scale bars:2μm(a-e); 500nm (i-k).