Figure 1

bSOFI algorithm. Flowchart to illustrate the different steps of the bSOFI algorithm. (a) Raw data. (b) Cross-cumulant computation up to order n according to equation (1) without time lags. (c) Cumulant ratios K ₁ and K ₂ according to equation (6). (d) Deconvolved cumulant of order n. (e) Solution for the spatial distribution of the molecular brightness ε, on-time ratio ρ _onand density N using equations (7-9). (f) Balanced cumulant of order n, obtained by computing the n-th root of the deconvolved cumulant, reconvolving with $U\left(n\overrightarrow{r}\right)$ and filling in the structural gaps around the roots of f _n with a lower-order cumulant. (g) Color-coded molecular parameter maps overlaid with a balanced cumulant as a transparency mask.