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  1. Optical nanoscopy based on separation of single molecules by stochastic switching and subsequent localization allows surpassing the diffraction limit of light. The growing pursuit towards live-cell imaging usi...

    Authors: Venkataraman Krishnaswami, Cornelis J F Van Noorden, Erik M M Manders and Ron A Hoebe
    Citation: Optical Nanoscopy 2014 3:1
  2. Heterochromatin in the nucleus of human embryonic cells plays an important role in the epigenetic regulation of gene expression. The architecture of heterochromatin and its dynamic organization remain elusive ...

    Authors: Ying S Hu, Quan Zhu, Keri Elkins, Kevin Tse, Yu Li, James A J Fitzpatrick, Inder M Verma and Hu Cang
    Citation: Optical Nanoscopy 2013 2:7
  3. The dimensions of neuronal synapses suggest that optical super-resolution imaging methods are necessary for thorough investigation of protein distributions and interactions. Nanoscopic evaluation of neuronal s...

    Authors: Thomas Liebmann, Hans Blom, Anita Aperia and Hjalmar Brismar
    Citation: Optical Nanoscopy 2013 2:6
  4. To enhance the resolution of a confocal laser scanning microscope the additional information of a pinhole plane image taken at every excitation scan position can be used (Sheppard 1988). This photon reassignme...

    Authors: Stephan Roth, Colin JR Sheppard, Kai Wicker and Rainer Heintzmann
    Citation: Optical Nanoscopy 2013 2:5
  5. Novel microscopic techniques which bypass the resolution limit in light microscopy are becoming routinely established today. The higher spatial resolution of super-resolution microscopy techniques demands for ...

    Authors: Patrick JM Zessin, Carmen L Krüger, Sebastian Malkusch, Ulrike Endesfelder and Mike Heilemann
    Citation: Optical Nanoscopy 2013 2:4
  6. Multi-color super-resolution (SR) imaging microscopy techniques can resolve ultrastructural relationships between- and provide co-localization information of- different proteins inside the cell or even within ...

    Authors: Maria Elena Gallina, Jianmin Xu, Thomas Dertinger, Adva Aizer, Yaron Shav-Tal and Shimon Weiss
    Citation: Optical Nanoscopy 2013 2:2
  7. Progress in nanosciences and life sciences is closely related to developments of high resolution imaging techniques. We introduce a technique which produces correlated topography and fluorescence lifetime imag...

    Authors: Olaf Schulz, Zhao Zhao, Alex Ward, Marcelle Koenig, Felix Koberling, Yan Liu, Jörg Enderlein, Hao Yan and Robert Ros
    Citation: Optical Nanoscopy 2013 2:1
  8. This paper analyses the resolution achieved in localisation microscopy experiments. The resolution is an essential metric for the correct interpretation of super-resolution images, but it varies between specim...

    Authors: Eric J Rees, Miklos Erdelyi, Dorothea Pinotsi, Alex Knight, Daniel Metcalf and Clemens F Kaminski
    Citation: Optical Nanoscopy 2012 1:12
  9. We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. pro...

    Authors: Matthias Bartels, Marius Priebe, Robin N Wilke, Sven P Krüger, Klaus Giewekemeyer, Sebastian Kalbfleisch, Christian Olendrowitz, Michael Sprung and Tim Salditt
    Citation: Optical Nanoscopy 2012 1:10
  10. This work explores the potential of multi-color Photoactivated Localization Microscopy (PALM) imaging to probe sub-diffraction limit interactions between proteins with spectrally separated labels. Using a PALM...

    Authors: Paolo Annibale, Marco Scarselli, Mattia Greco and Aleksandra Radenovic
    Citation: Optical Nanoscopy 2012 1:9
  11. In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent ...

    Authors: Christian A Wurm, Kirill Kolmakov, Fabian Göttfert, Haisen Ta, Mariano Bossi, Heiko Schill, Sebastian Berning, Stefan Jakobs, Gerald Donnert, Vladimir N Belov and Stefan W Hell
    Citation: Optical Nanoscopy 2012 1:7
  12. We present a new instrument for nanoscopic investigations by coupling an atomic force microscope (AFM) with a super resolution stimulated emission depletion (STED) microscope. This nanoscopic tool allows high ...

    Authors: Benjamin Harke, Jenu Varghese Chacko, Heiko Haschke, Claudio Canale and Alberto Diaspro
    Citation: Optical Nanoscopy 2012 1:3
  13. Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to local...

    Authors: Stefan Geissbuehler, Noelia L Bocchio, Claudio Dellagiacoma, Corinne Berclaz, Marcel Leutenegger and Theo Lasser
    Citation: Optical Nanoscopy 2012 1:4
  14. Fluorescence-based biological imaging has been revolutionized by the recent introduction of superresolution microscopy methods. 3D superresolution microscopy, however, remains a challenge as its implementation...

    Authors: Thomas Dertinger, Jianmin Xu, Omeed Foroutan Naini, Robert Vogel and Shimon Weiss
    Citation: Optical Nanoscopy 2012 1:2